![]() To increase the range of CO2 concentrations, we adjusted the outdoor air supply rates every 3 months. Indoor carbon dioxide (CO2 concentrations were recorded every 10 minutes as a surrogate for outdoor air supply. Nasal lavage samples from building occupants with upper respiratory infections were also collected. We directly extracted RNA from the filters for nested reverse transcriptase-polymerase chain reaction analysis of rhinovirus. each workday, with each sample run for 1 work week. We investigated detection of airborne rhinovirus in office environments by polymerase chain reaction technology and related detection to outdoor air supply rates. Rhinoviruses are major causes of morbidity in patients with respiratory diseases however, their modes of transmission are controversial.
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